Analysis Note

>=75% inhibition with active-site titrated porcine trypsin using benzoyl-Arg-p-nitroanilide (Bz-Arg-pNA) as the substrate.

Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary Targetserine proteases

General description

A single polypeptide chain of 394 amino acids and three carbohydrate side chains linked to asparagine residues present at about 290 mg/100 ml in acute phase plasma. Serine protease inhibitor that also acts as a major physiological regulator of elastase. Inhibitor of neutrophil elastase, cathepsin G, and proteinase 3.

A single polypeptide chain of 394 amino acids and three carbohydrate side chains linked to asparagine residues. Present at about 290 mg/100 ml in acute phase plasma. Serine protease inhibitor that also acts as a major physiological regulator of elastase.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Coakley, R.J., et al. 2001. Am. J. Med. Sci.321, 33.Travis, J., and Salvesen, G.S. 1983. Annu. Rev. Biochem.52, 655.

Packaging

1 mg in Glass bottle

5 mg in Plastic ampoule

Physical form

Lyophilized from 300 mM NaCl, 30 mM sodium phosphate buffer, pH 6.5.

Preparation Note

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Please refer to vial label for lot-specific volume prior to lyophilization.

Reconstitution

Following reconstitution aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.

Warning

Toxicity: Standard Handling (A)